Hotspot

ycf1

SSC · 128506-134088 · 5583 bp

Back to Cuphea

Pi 0.0507
MarkerSeek score 73.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5583
variable_sites
718
pi
0.0507
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TTTCGGGGAGAACCAAAAGG AGTTCCTCGATGGTCGTACA 50.0 50.0 58.0 58.2 918-924 bp 0.857143 81.6
ycf1_p2 TTTCGGGGAGAACCAAAAGG ACACGGAAATGGGTTGGATC 50.0 50.0 58.0 58.2 1198 bp 0.142857 44.3
ycf1_p3 TTTCGGGGAGAACCAAAAGG GACACGGAAATGGGTTGGAT 50.0 50.0 58.0 58.2 1199 bp 0.142857 44.3
ycf1_p4 TTTCGGGGAGAACCAAAAGG CACGGAAATGGGTTGGATCA 50.0 50.0 58.0 58.2 1197 bp 0.142857 44.3
ycf1_p5 AGTCTTCGGACCATTCAGGA ACACGGAAATGGGTTGGATC 50.0 50.0 58.1 58.2 933 bp 0.142857 43.9