Hotspot

ndhF-rpl32

SSC · 116575-117608 · 1034 bp

Back to Cuphea

Pi 0.088
MarkerSeek score 87.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1034
variable_sites
197
pi
0.088
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACTGGTAGTGGAATGAAAGGT TTTGATATAGAAGTGCGTTTTT 42.9 27.3 56.5 52.4 1082-1223 bp 1.0 52.0
ndhF-rpl32_p2 ACTGGTAGTGGAATGAAAGGT TTTTGATATAGAAGTGCGTTTT 42.9 27.3 56.5 52.4 1083-1224 bp 1.0 52.0
ndhF-rpl32_p3 ACTGGTAGTGGAATGAAAGGT TTTTTGATATAGAAGTGCGTTT 42.9 27.3 56.5 52.4 1084-1225 bp 1.0 52.0
ndhF-rpl32_p4 AGGAACTGGTAGTGGAATGA TTTGATATAGAAGTGCGTTTTT 45.0 27.3 55.5 52.4 1086-1227 bp 1.0 52.0
ndhF-rpl32_p5 AGGAACTGGTAGTGGAATGA TTTTGATATAGAAGTGCGTTTT 45.0 27.3 55.5 52.4 1087-1228 bp 1.0 52.0