Hotspot

accD-psaI

LSC · 62485-63177 · 693 bp

Pi 0.0533

MarkerSeek score 84.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 91
pi: 0.0533
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGCCGAACCTAATGCCTAC	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.0	57.9	977-1031 bp	1.0	90.1
accD-psaI_p2	TTGCCGAACCTAATGCCTAC	TAGCCACTACCCACATTCCA	50.0	50.0	58.0	58.0	1612-1811 bp	1.0	89.1
accD-psaI_p3	GTTTTGGGATGTTGGGGGAT	TAGCCACTACCCACATTCCA	50.0	50.0	58.0	58.0	1639-1838 bp	1.0	88.8
accD-psaI_p4	GCCTACATTGCATTTGCTGG	TAGCCACTACCCACATTCCA	50.0	50.0	58.1	58.0	1598-1797 bp	1.0	88.8
accD-psaI_p5	GCGCTTGATCGAGTATGCTA	TAGCCACTACCCACATTCCA	50.0	50.0	57.9	58.0	1839-2038 bp	1.0	88.3