Hotspot

ndhC-trnV-UAC

LSC · 53625-54928 · 1304 bp

Back to Cuphea

Pi 0.052
MarkerSeek score 85.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1304
variable_sites
166
pi
0.052
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AAAGTTTCTCTGGGCCCTTG GTAATGCTCCTGAGTCGCAA 50.0 50.0 58.0 58.0 1655-1756 bp 0.857143 85.7
ndhC-trnV-UAC_p2 AAAGTTTCTCTGGGCCCTTG TCGCCCGATGAACTTTAAGG 50.0 50.0 58.0 58.0 1797-1897 bp 0.857143 85.4
ndhC-trnV-UAC_p3 AAAGTTTCTCTGGGCCCTTG TGAATCAGAGCACATGGAGC 50.0 50.0 58.0 58.0 1626-1635 bp 0.428571 64.0
ndhC-trnV-UAC_p4 TTGAAAGTTTCTCTGGGCCC GTAATGCTCCTGAGTCGCAA 50.0 50.0 58.0 58.0 1658 bp 0.142857 45.6
ndhC-trnV-UAC_p5 TTGAAAGTTTCTCTGGGCCC TCGCCCGATGAACTTTAAGG 50.0 50.0 58.0 58.0 1800 bp 0.142857 45.5