Hotspot

trnF-GAA-ndhJ

LSC · 51207-51940 · 734 bp

Back to Cuphea

Pi 0.0487
MarkerSeek score 83.90
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
734
variable_sites
88
pi
0.0487
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AAAGATGCAGGGTCGTTTGT 55.0 45.0 58.0 58.0 1276-1341 bp 1.0 90.1
trnF-GAA-ndhJ_p2 ACGGCGCGAATCTCTATTTT AAAGATGCAGGGTCGTTTGT 45.0 45.0 58.1 58.0 1909-1958 bp 1.0 90.1
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA AAAGATGCAGGGTCGTTTGT 50.0 45.0 58.1 58.0 1240-1305 bp 1.0 90.0
trnF-GAA-ndhJ_p4 ATTAATGACGGCGCGAATCT AAAGATGCAGGGTCGTTTGT 45.0 45.0 58.1 58.0 1916-1965 bp 1.0 89.5
trnF-GAA-ndhJ_p5 TGCCTAAGTAATGGTCGGGA AAAGATGCAGGGTCGTTTGT 50.0 45.0 58.1 58.0 1292-1357 bp 1.0 89.0