Hotspot

psbZ-trnG-GCC

LSC · 38971-39390 · 420 bp

Back to Cuphea

Pi 0.0797
MarkerSeek score 76.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
420
variable_sites
68
pi
0.0797
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.88
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 GCGGATAGGGGGATCAAATG CGTTGTTTTTCGACCGGAAG 55.0 50.0 58.2 58.0 1207-1361 bp 1.0 90.5
psbZ-trnG-GCC_p2 TTTTCAAGACCGGAGCCATC CGTTGTTTTTCGACCGGAAG 50.0 50.0 58.2 58.0 1543-1714 bp 1.0 90.5
psbZ-trnG-GCC_p3 GCGGATAGGGGGATCAAATG CTTGAGGTCACGGGTTCAAA 55.0 50.0 58.2 58.0 884-1033 bp 1.0 90.3
psbZ-trnG-GCC_p4 GCGGATAGGGGGATCAAATG GTGAAATGGTTCAGGGGTGT 55.0 50.0 58.2 58.0 1210 bp 0.142857 44.3
psbZ-trnG-GCC_p5 TTTTCAAGACCGGAGCCATC GTGAAATGGTTCAGGGGTGT 50.0 50.0 58.2 58.0 1531 bp 0.142857 44.2