Hotspot

trnT-GGU-psbD

LSC · 34228-35679 · 1452 bp

Back to Cuphea

Pi 0.0532
MarkerSeek score 85.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1452
variable_sites
178
pi
0.0532
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TGGACCAGAGAATGCGATTG 55.0 50.0 58.1 58.0 1943-1988 bp 1.0 91.1
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TGGACCAGAGAATGCGATTG 50.0 50.0 58.1 58.0 1946-1991 bp 1.0 90.9
trnT-GGU-psbD_p3 GCATAGTCGTGGCCCATTTA TGGACCAGAGAATGCGATTG 50.0 50.0 58.0 58.0 2016 bp 0.142857 45.2
trnT-GGU-psbD_p4 GCATAGTCGTGGCCCATTTA CCAGACTGGCCTAGTGGATA 50.0 55.0 58.0 57.9 2066 bp 0.142857 44.7
trnT-GGU-psbD_p5 GCATAGTCGTGGCCCATTTA GCACGGAATGTATTTGCACC 50.0 50.0 58.0 58.1 2270 bp 0.142857 44.7