Hotspot

trnE-UUC-trnT-GGU

LSC · 33152-34155 · 1004 bp

Back to Cuphea

Pi 0.077
MarkerSeek score 82.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1004
variable_sites
180
pi
0.077
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGACCCAGGACGAATCAATT GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1149-1205 bp 1.0 91.8
trnE-UUC-trnT-GGU_p2 CCCCCTCCTTGAAAGAGAGA GAACCGATGACTTACGCCTT 55.0 50.0 58.0 58.0 1058-1106 bp 1.0 91.6
trnE-UUC-trnT-GGU_p3 CGACCCAGGACGAATCAATT CCATGGCGTTACTCTACCAC 50.0 55.0 58.0 58.1 1128-1184 bp 1.0 91.3
trnE-UUC-trnT-GGU_p4 CCCCCTCCTTGAAAGAGAGA CCATGGCGTTACTCTACCAC 55.0 55.0 58.0 58.1 1037-1085 bp 1.0 91.2
trnE-UUC-trnT-GGU_p5 CGACCCAGGACGAATCAATT TGGCGTTACTCTACCACTGA 50.0 50.0 58.0 58.1 1125-1181 bp 1.0 91.1