Hotspot

psbM-trnD-GUC

LSC · 31370-32448 · 1079 bp

Back to Cuphea

Pi 0.0534
MarkerSeek score 88.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1079
variable_sites
141
pi
0.0534
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GGAACTAGAATGAAAAGTGCAGT AGTTCAATGGGTCAGAGCAC 39.1 50.0 57.1 57.8 1159-1221 bp 1.0 58.9
psbM-trnD-GUC_p2 AGAATGAAAAGTGCAGTAGC AGTTCAATGGGTCAGAGCAC 40.0 50.0 53.9 57.8 1153-1215 bp 1.0 57.8
psbM-trnD-GUC_p3 ACTAGAATGAAAAGTGCAGTAGC AGTTCAATGGGTCAGAGCAC 39.1 50.0 56.9 57.8 1156-1218 bp 1.0 57.5
psbM-trnD-GUC_p4 AGGAACTAGAATGAAAAGTGCAG AGTTCAATGGGTCAGAGCAC 39.1 50.0 56.8 57.8 1160-1222 bp 1.0 56.8
psbM-trnD-GUC_p5 AGGAACTAGAATGAAAAGTGCA AGTTCAATGGGTCAGAGCAC 36.4 50.0 55.6 57.8 1160-1222 bp 1.0 55.6