Hotspot

petN-psbM

LSC · 30311-31264 · 954 bp

Back to Cuphea

Pi 0.0661
MarkerSeek score 83.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
954
variable_sites
153
pi
0.0661
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GAAAGGGCGGAAGATACCAG AGAAAGGGGTTGGATCAGGA 55.0 50.0 58.0 57.9 1979-2100 bp 0.714286 77.0
petN-psbM_p2 GAAAGGGCGGAAGATACCAG CCCAACTTCTTAGAGGGGGA 55.0 55.0 58.0 58.0 2261 bp 0.142857 45.1
petN-psbM_p3 GAAAGGGCGGAAGATACCAG TCCCAACTTCTTAGAGGGGG 55.0 55.0 58.0 58.0 2262 bp 0.142857 45.1
petN-psbM_p4 GAAAGGGCGGAAGATACCAG GGAACTGGAAGGGCGTTAAA 55.0 50.0 58.0 58.1 2110 bp 0.142857 44.6
petN-psbM_p5 GGGTCACTGCCTATTTTGCT CCCAACTTCTTAGAGGGGGA 50.0 55.0 58.2 58.0 1884 bp 0.142857 44.2