Hotspot

trnC-GCA-petN

LSC · 29308-30220 · 913 bp

Back to Cuphea

Pi 0.0491
MarkerSeek score 88.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
913
variable_sites
103
pi
0.0491
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 707-976 bp 1.0 89.8
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG CTACCATTAACGCAGCCCAA 50.0 50.0 58.1 58.3 719-988 bp 1.0 89.3
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG TACCATTAACGCAGCCCAAG 50.0 50.0 58.1 58.3 718-987 bp 1.0 89.3
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGACTACCATTAACGCAGCC 50.0 50.0 58.1 57.7 722-991 bp 1.0 88.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 767-1036 bp 1.0 88.5