Hotspot

atpH-atpI

LSC · 14830-15880 · 1051 bp

Back to Cuphea

Pi 0.0706
MarkerSeek score 84.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1051
variable_sites
176
pi
0.0706
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TCATGGGGAATTAGCTGCAC 45.0 50.0 58.0 57.9 1406-1593 bp 1.0 91.2
atpH-atpI_p2 GAAGCAAGTCCGACAGCTAA TCATGGGGAATTAGCTGCAC 50.0 50.0 57.9 57.9 1436-1623 bp 1.0 90.9
atpH-atpI_p3 TACCTTGACCAACTCCAGGT TCATGGGGAATTAGCTGCAC 50.0 50.0 57.9 57.9 1462-1649 bp 1.0 90.6
atpH-atpI_p4 TCCAGGTCCGATAGAAGCAA TCATGGGGAATTAGCTGCAC 50.0 50.0 58.1 57.9 1449-1636 bp 1.0 90.4
atpH-atpI_p5 ATAACGGAAGCGGCAGAAAT AATTAGCTGCACCCACGAAT 45.0 45.0 58.0 57.9 1398-1585 bp 0.857143 84.9