Hotspot

atpF-atpH

LSC · 14039-14583 · 545 bp

Back to Cuphea

Pi 0.0639
MarkerSeek score 84.80
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
545
variable_sites
82
pi
0.0639
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GAAAGAATCGGTTACATTTTTCA ATTTGCGAATCCTTTTGTGT 30.4 35.0 53.9 54.4 590-643 bp 1.0 52.0
atpF-atpH_p2 AAAGAATCGGTTACATTTTTCA ATTTGCGAATCCTTTTGTGT 27.3 35.0 52.6 54.4 589-642 bp 1.0 52.0
atpF-atpH_p3 GAAAGAATCGGTTACATTTTTCAT ATTTGCGAATCCTTTTGTGT 29.2 35.0 54.5 54.4 590-643 bp 1.0 52.0
atpF-atpH_p4 AAGAATCGGTTACATTTTTCAT ATTTGCGAATCCTTTTGTGT 27.3 35.0 52.4 54.4 588-641 bp 1.0 52.0
atpF-atpH_p5 GAAAGAATCGGTTACATTTTTCA TTGCGAATCCTTTTGTGTAA 30.4 35.0 53.9 53.7 588-641 bp 1.0 52.0