Hotspot

trnS-GCU-trnG-UCC

LSC · 8975-9751 · 777 bp

Back to Cuphea

Pi 0.0595
MarkerSeek score 85.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
777
variable_sites
104
pi
0.0595
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 815-861 bp 1.0 63.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 814-860 bp 1.0 63.7
trnS-GCU-trnG-UCC_p3 GCTCGTACAACGGATTAGCA ACGAATCACACTTTTACCACT 50.0 38.1 58.1 55.5 833-879 bp 1.0 63.1
trnS-GCU-trnG-UCC_p4 AGCTCGTACAACGGATTAGC ACGAATCACACTTTTACCACT 50.0 38.1 57.8 55.5 834-880 bp 1.0 62.1
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 816-862 bp 1.0 61.8