Hotspot

rps16-trnQ-UUG

LSC · 6435-7560 · 1126 bp

Back to Cuphea

Pi 0.0754
MarkerSeek score 84.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1126
variable_sites
201
pi
0.0754
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1194-1553 bp 1.0 89.8
rps16-trnQ-UUG_p2 GCACGTTGCTTTTTACCACA GGGTTTTGGTCCCGGTATTC 45.0 55.0 57.8 58.5 1215-1574 bp 1.0 86.2
rps16-trnQ-UUG_p3 GCACGTTGCTTTTTACCACA TTCGGAGGTTCGAATCCTTC 45.0 50.0 57.8 57.3 1198-1557 bp 1.0 85.1
rps16-trnQ-UUG_p4 GCACGTTGCTTTTTACCACA TAAGGCAACGGGTTTTGGTC 45.0 50.0 57.8 58.7 1224-1583 bp 1.0 85.0
rps16-trnQ-UUG_p5 CGCACGTTGCTTTTTACCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1195-1554 bp 1.0 84.3