Hotspot

trnH-GUG-psbA

LSC · 81-504 · 424 bp

Back to Cuphea

Pi 0.128
MarkerSeek score 79.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
424
variable_sites
120
pi
0.128
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.91
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCGAAGCTCCATCTACAAATGG 55.0 45.5 58.1 58.2 429-584 bp 1.0 73.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 443-598 bp 1.0 73.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 442-597 bp 1.0 73.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TCGAAGCTCCATCTACAAATGG 50.0 45.5 57.9 58.2 444-599 bp 1.0 73.3
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CGAAGCTCCATCTACAAATGG 55.0 47.6 58.1 56.8 428-583 bp 1.0 73.1