Hotspot

ycf2

Genome · 61529-68563 · 7035 bp

Back to Cunninghamia

Pi 0
MarkerSeek score 55.20
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
Genome
length_bp
7035
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 GAATAAGTTGTATTAACTATGTACTTC AGCATCTTGTCGCCTTTTCA 25.9 45.0 52.7 58.1 1161 bp 1.0 40.0
ycf2_p2 GAATAAGTTGTATTAACTATGTACTTC TGAAAAGCATCTTGTCGCCT 25.9 45.0 52.7 58.1 1166 bp 1.0 40.0
ycf2_p3 GAATAAGTTGTATTAACTATGTACTTC GCATCTTGTCGCCTTTTCAA 25.9 45.0 52.7 57.3 1160 bp 1.0 40.0
ycf2_p4 GAATAAGTTGTATTAACTATGTACTTC TTGAAAAGCATCTTGTCGCC 25.9 45.0 52.7 57.3 1167 bp 1.0 40.0
ycf2_p5 GAATAAGTTGTATTAACTATGTACTTC AAGCATCTTGTCGCCTTTTC 25.9 45.0 52.7 57.0 1162 bp 1.0 40.0