Hotspot

rps2

Genome · 36802-37497 · 696 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 27.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: Genome
length_bp: 696
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GGTTGAAGGGCTTGAAGGAA	CCCAAGGAAAAAGAGCACCT	50.0	50.0	58.0	58.0	1758 bp	1.0	80.0
rps2_p2	GGTTGAAGGGCTTGAAGGAA	AGCCAAGTCCCGAACAAATT	50.0	45.0	58.0	58.0	1652 bp	1.0	80.0
rps2_p3	GGTTGAAGGGCTTGAAGGAA	TCCCCAAGGAAAAAGAGCAC	50.0	50.0	58.0	58.0	1760 bp	1.0	80.0
rps2_p4	GGTTGAAGGGCTTGAAGGAA	TTGTAGGTGCGGCTAATTCC	50.0	50.0	58.0	58.0	1800 bp	1.0	79.7
rps2_p5	ACCTGTCGGTACTGGATTCA	CCCAAGGAAAAAGAGCACCT	50.0	50.0	58.1	58.0	1715 bp	1.0	79.5