Hotspot

trnF-accD

Genome · 118188-119938 · 1751 bp

Back to Cunninghamia

Pi 0.000571

MarkerSeek score 58.60

Primer available yes

Label trnF-accD

Feature Metadata

label_name: trnF-accD
feature_type: igs
region: Genome
length_bp: 1751
variable_sites: 1
pi: 0.000571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-accD_p1	GCTAGTATGATGCACAGGGG	GGTTCCACGAGCTCTTTTGA	55.0	50.0	57.8	58.1	1909-1910 bp	1.0	77.7
trnF-accD_p2	GCTAGTATGATGCACAGGGG	CATGGTTCCACGAGCTCTTT	55.0	50.0	57.8	58.2	1912-1913 bp	1.0	77.1
trnF-accD_p3	GTATGATGCACAGGGGAACA	GGTTCCACGAGCTCTTTTGA	50.0	50.0	57.6	58.1	1905-1906 bp	1.0	75.7
trnF-accD_p4	GCTAGTATGATGCACAGGGG	TGGTTCCACGAGCTCTTTTG	55.0	50.0	57.8	58.4	1910-1911 bp	1.0	75.4
trnF-accD_p5	GTATGATGCACAGGGGAACA	CATGGTTCCACGAGCTCTTT	50.0	50.0	57.6	58.2	1908-1909 bp	1.0	75.2