Hotspot

psaI-ycf4

Genome · 83845-84445 · 601 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 58.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: Genome
length_bp: 601
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TTACCCTCTGTTTTCGTGCC	GAGCCATTTGGATCGACGAT	50.0	50.0	58.1	58.1	721 bp	1.0	78.2
psaI-ycf4_p2	CTGTTTTCGTGCCTTTGGTG	GAGCCATTTGGATCGACGAT	50.0	50.0	58.2	58.1	714 bp	1.0	77.8
psaI-ycf4_p3	TCTGTTTTCGTGCCTTTGGT	GAGCCATTTGGATCGACGAT	45.0	50.0	58.2	58.1	715 bp	1.0	77.1
psaI-ycf4_p4	TTACCCTCTGTTTTCGTGCC	AGCCATTTGGATCGACGATT	50.0	45.0	58.1	57.7	720 bp	1.0	76.5
psaI-ycf4_p5	CTGTTTTCGTGCCTTTGGTG	AGCCATTTGGATCGACGATT	50.0	45.0	58.2	57.7	713 bp	1.0	76.2