Hotspot

trnI-ycf2

Genome · 60885-61528 · 644 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 58.90

Primer available yes

Label trnI-ycf2

Feature Metadata

label_name: trnI-ycf2
feature_type: igs
region: Genome
length_bp: 644
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-ycf2_p1	TACCATTCAGCCATGGATGC	GGTTCGTTTCGATAGGGTCC	50.0	55.0	58.0	58.1	712-713 bp	1.0	79.5
trnI-ycf2_p2	CCGCGACTTCCCAATTATGA	GGTTCGTTTCGATAGGGTCC	50.0	55.0	58.1	58.1	744-745 bp	1.0	79.1
trnI-ycf2_p3	TACCATTCAGCCATGGATGC	ATAGGGTCCATAGGCAACGT	50.0	50.0	58.0	58.2	701-702 bp	1.0	78.4
trnI-ycf2_p4	TACCATTCAGCCATGGATGC	TCTGTTTCGGTTCGTTTCGA	50.0	45.0	58.0	57.8	720-721 bp	1.0	78.3
trnI-ycf2_p5	CCGCGACTTCCCAATTATGA	ATAGGGTCCATAGGCAACGT	50.0	50.0	58.1	58.2	733-734 bp	1.0	78.1