Hotspot

rps2-atpI

Genome · 37498-37743 · 246 bp

Back to Cunninghamia

Pi 0.00407

MarkerSeek score 54.80

Primer available yes

Label rps2-atpI

Feature Metadata

label_name: rps2-atpI
feature_type: igs
region: Genome
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-atpI_p1	ATTGTGACCCAGATCTCGTG	TTCCACACCGGATATCTCGT	50.0	50.0	57.3	58.2	425 bp	1.0	72.9
rps2-atpI_p2	TGACCCAGATCTCGTGGATA	TTCCACACCGGATATCTCGT	50.0	50.0	57.3	58.2	421 bp	1.0	72.4
rps2-atpI_p3	TTCCAGCCAATGATGATGGT	TTCCACACCGGATATCTCGT	45.0	50.0	57.2	58.2	395 bp	1.0	71.7
rps2-atpI_p4	TCCCAATTCCAGCCAATGAT	TTCCACACCGGATATCTCGT	45.0	50.0	57.1	58.2	401 bp	1.0	71.6
rps2-atpI_p5	ATCCCAATTCCAGCCAATGA	TTCCACACCGGATATCTCGT	45.0	50.0	57.1	58.2	402 bp	1.0	71.5