Hotspot

psbD-trnT

Genome · 21090-22487 · 1398 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 59.30

Primer available yes

Label psbD-trnT

Feature Metadata

label_name: psbD-trnT
feature_type: igs
region: Genome
length_bp: 1398
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT_p1	AAGCAATAGACCGGACCAAC	GCCCTTTTAACTCAGCGGTA	50.0	50.0	57.9	57.9	1576-1584 bp	1.0	78.7
psbD-trnT_p2	ACAAAACGGTCTCTGCGTAG	GCCCTTTTAACTCAGCGGTA	50.0	50.0	58.2	57.9	1548-1556 bp	1.0	77.8
psbD-trnT_p3	AACAAAACGGTCTCTGCGTA	GCCCTTTTAACTCAGCGGTA	45.0	50.0	57.8	57.9	1549-1557 bp	1.0	77.8
psbD-trnT_p4	AAGCAATAGACCGGACCAAC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	57.9	58.3	1539-1547 bp	1.0	77.3
psbD-trnT_p5	ACAAAACGGTCTCTGCGTAG	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	1511-1519 bp	1.0	76.4