Hotspot

trnK-rps16

Genome · 4023-4737 · 715 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: Genome
length_bp: 715
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AGTACTTCATCCGAACGTGC	TTAGGAGCTCAACCTACGGG	50.0	55.0	58.0	58.5	892-893 bp	1.0	75.8
trnK-rps16_p2	CATCCGAACGTGCAAAAGAC	TTAGGAGCTCAACCTACGGG	50.0	55.0	58.0	58.5	885-886 bp	1.0	75.5
trnK-rps16_p3	TCATCCGAACGTGCAAAAGA	TTAGGAGCTCAACCTACGGG	45.0	55.0	58.1	58.5	886-887 bp	1.0	74.8
trnK-rps16_p4	GTACTTCATCCGAACGTGCA	TTAGGAGCTCAACCTACGGG	50.0	55.0	58.3	58.5	891-892 bp	1.0	73.5
trnK-rps16_p5	ACTTCATCCGAACGTGCAAA	TTAGGAGCTCAACCTACGGG	45.0	55.0	58.4	58.5	889-890 bp	1.0	72.6