primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TGGCCAAGCAGCTAAAAAGA	TGCGATTGGATCACTAGCAC	20	20	45.0	50.0	58.0	58.1	0.6	0.0	0.066070	301	900	786-786	1.000000	79.5
psbA_p2	psbA	2	CTACTGGCCAAGCAGCTAAA	TGCGATTGGATCACTAGCAC	20	20	50.0	50.0	57.9	58.1	7.0	0.0	0.173886	301	900	790-790	1.000000	78.6
psbA_p3	psbA	3	CATAGTGCTGATGCCCAGAG	TGCGATTGGATCACTAGCAC	20	20	55.0	50.0	58.1	58.1	7.3	0.0	0.179755	301	900	828-828	1.000000	78.6
psbA_p4	psbA	4	TACTACTGGCCAAGCAGCTA	TGCGATTGGATCACTAGCAC	20	20	50.0	50.0	58.1	58.1	7.0	0.0	0.199385	301	900	792-792	1.000000	78.4
psbA_p5	psbA	5	TGGCCAAGCAGCTAAAAAGA	GCGATTGGATCACTAGCACT	20	20	45.0	50.0	58.0	57.8	0.6	0.0	0.237259	301	900	785-785	1.000000	78.1
matK-trnK_p1	matK-trnK	1	TGCGTAAAGATCTTCCCGAA	ACTGCGTATTCCACTTCGAC	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3289	3985	861-861	1.000000	71.6
matK-trnK_p2	matK-trnK	2	TGCGTAAAGATCTTCCCGAA	GCGTATTCCACTTCGACTGT	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3289	3985	858-858	1.000000	71.6
matK-trnK_p3	matK-trnK	3	TTGCGTAAAGATCTTCCCGA	ACTGCGTATTCCACTTCGAC	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3289	3985	862-862	1.000000	71.6
matK-trnK_p4	matK-trnK	4	TTGCGTAAAGATCTTCCCGA	GCGTATTCCACTTCGACTGT	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.050404	3289	3985	859-859	1.000000	71.6
matK-trnK_p5	matK-trnK	5	TGCGTAAAGATCTTCCCGAA	TGCGTATTCCACTTCGACTG	20	20	45.0	50.0	57.0	58.0	23.3	0.0	1.051774	3289	3985	859-859	1.000000	71.6
trnK-rps16_p1	trnK-rps16	1	AGTACTTCATCCGAACGTGC	TTAGGAGCTCAACCTACGGG	20	20	50.0	55.0	58.0	58.5	0.0	17.2	0.525959	4023	4737	892-893	1.000000	75.8
trnK-rps16_p2	trnK-rps16	2	CATCCGAACGTGCAAAAGAC	TTAGGAGCTCAACCTACGGG	20	20	50.0	55.0	58.0	58.5	0.0	17.2	0.567657	4023	4737	885-886	1.000000	75.5
trnK-rps16_p3	trnK-rps16	3	TCATCCGAACGTGCAAAAGA	TTAGGAGCTCAACCTACGGG	20	20	45.0	55.0	58.1	58.5	0.0	17.2	0.645741	4023	4737	886-887	1.000000	74.8
trnK-rps16_p4	trnK-rps16	4	GTACTTCATCCGAACGTGCA	TTAGGAGCTCAACCTACGGG	20	20	50.0	55.0	58.3	58.5	0.0	17.2	0.812873	4023	4737	891-892	1.000000	73.5
trnK-rps16_p5	trnK-rps16	5	ACTTCATCCGAACGTGCAAA	TTAGGAGCTCAACCTACGGG	20	20	45.0	55.0	58.4	58.5	0.0	17.2	0.929455	4023	4737	889-890	1.000000	72.6
rps16_p1	rps16	1	GGACCTAGACGTAGACGCAT	TCCATTTTGTATCAGATCAAAGA	20	23	55.0	30.4	58.7	53.7	1.1	0.0	7.958517	4738	5798	1115-1115	1.000000	40.0
rps16_p2	rps16	2	GGACCTAGACGTAGACGCAT	TCCATTTTGTATCAGATCAAAGATCT	20	26	55.0	30.8	58.7	56.7	1.1	3.8	7.968769	4738	5798	1115-1115	1.000000	40.0
rps16_p3	rps16	3	TTGGACCTAGACGTAGACGC	TCCATTTTGTATCAGATCAAAGA	20	23	55.0	30.4	58.9	53.7	1.1	0.0	8.175694	4738	5798	1117-1117	1.000000	40.0
rps16_p4	rps16	4	TTGGACCTAGACGTAGACGC	TCCATTTTGTATCAGATCAAAGATCT	20	26	55.0	30.8	58.9	56.7	1.1	3.8	8.185947	4738	5798	1117-1117	1.000000	40.0
rps16_p5	rps16	5	GGACCTAGACGTAGACGCAT	TCCATTTTGTATCAGATCAAAGATC	20	25	55.0	32.0	58.7	55.5	1.1	0.0	8.241278	4738	5798	1115-1115	1.000000	40.0
ycf3-psaA_p1	ycf3-psaA	1	TGGTTTGATCAAGCTGCTGA	AGAAATGACCGGGTTTAGCC	20	20	45.0	50.0	57.7	57.9	19.9	6.3	0.399411	11801	12409	813-814	1.000000	76.9
ycf3-psaA_p2	ycf3-psaA	2	TGGTTTGATCAAGCTGCTGA	GGATCCCTCTCCACTACGAT	20	20	45.0	55.0	57.7	57.7	19.9	0.0	0.571207	11801	12409	769-770	1.000000	75.5
ycf3-psaA_p3	ycf3-psaA	3	TGGAAACAAGCTATAGCGCT	AGAAATGACCGGGTTTAGCC	20	20	45.0	50.0	57.3	57.9	17.3	6.3	0.815686	11801	12409	789-790	1.000000	73.6
ycf3-psaA_p4	ycf3-psaA	4	TGGAAACAAGCTATAGCGCT	GGATCCCTCTCCACTACGAT	20	20	45.0	55.0	57.3	57.7	17.3	0.0	0.987482	11801	12409	745-746	1.000000	72.2
ycf3-psaA_p5	ycf3-psaA	5	GGTTTGATCAAGCTGCTGAG	AGAAATGACCGGGTTTAGCC	20	20	50.0	50.0	57.1	57.9	19.9	6.3	1.040015	11801	12409	812-813	1.000000	71.8
psbD-trnT_p1	psbD-trnT	1	AAGCAATAGACCGGACCAAC	GCCCTTTTAACTCAGCGGTA	20	20	50.0	50.0	57.9	57.9	9.5	0.0	0.212962	21090	22487	1576-1584	1.000000	78.7
psbD-trnT_p2	psbD-trnT	2	ACAAAACGGTCTCTGCGTAG	GCCCTTTTAACTCAGCGGTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.323968	21090	22487	1548-1556	1.000000	77.8
psbD-trnT_p3	psbD-trnT	3	AACAAAACGGTCTCTGCGTA	GCCCTTTTAACTCAGCGGTA	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.331675	21090	22487	1549-1557	1.000000	77.8
psbD-trnT_p4	psbD-trnT	4	AAGCAATAGACCGGACCAAC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	57.9	58.3	9.5	0.0	0.392231	21090	22487	1539-1547	1.000000	77.3
psbD-trnT_p5	psbD-trnT	5	ACAAAACGGTCTCTGCGTAG	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.503237	21090	22487	1511-1519	1.000000	76.4
rps2_p1	rps2	1	GGTTGAAGGGCTTGAAGGAA	CCCAAGGAAAAAGAGCACCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.005700	36802	37497	1758-1758	1.000000	80.0
rps2_p2	rps2	2	GGTTGAAGGGCTTGAAGGAA	AGCCAAGTCCCGAACAAATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.009247	36802	37497	1652-1652	1.000000	80.0
rps2_p3	rps2	3	GGTTGAAGGGCTTGAAGGAA	TCCCCAAGGAAAAAGAGCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009247	36802	37497	1760-1760	1.000000	80.0
rps2_p4	rps2	4	GGTTGAAGGGCTTGAAGGAA	TTGTAGGTGCGGCTAATTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037304	36802	37497	1800-1800	1.000000	79.7
rps2_p5	rps2	5	ACCTGTCGGTACTGGATTCA	CCCAAGGAAAAAGAGCACCT	20	20	50.0	50.0	58.1	58.0	0.9	0.0	0.068054	36802	37497	1715-1715	1.000000	79.5
rps2-atpI_p1	rps2-atpI	1	ATTGTGACCCAGATCTCGTG	TTCCACACCGGATATCTCGT	20	20	50.0	50.0	57.3	58.2	0.0	9.5	0.908747	37498	37743	425-425	1.000000	72.9
rps2-atpI_p2	rps2-atpI	2	TGACCCAGATCTCGTGGATA	TTCCACACCGGATATCTCGT	20	20	50.0	50.0	57.3	58.2	9.0	9.5	0.976644	37498	37743	421-421	1.000000	72.4
rps2-atpI_p3	rps2-atpI	3	TTCCAGCCAATGATGATGGT	TTCCACACCGGATATCTCGT	20	20	45.0	50.0	57.2	58.2	0.0	9.5	1.061079	37498	37743	395-395	1.000000	71.7
rps2-atpI_p4	rps2-atpI	4	TCCCAATTCCAGCCAATGAT	TTCCACACCGGATATCTCGT	20	20	45.0	50.0	57.1	58.2	0.0	9.5	1.081149	37498	37743	401-401	1.000000	71.6
rps2-atpI_p5	rps2-atpI	5	ATCCCAATTCCAGCCAATGA	TTCCACACCGGATATCTCGT	20	20	45.0	50.0	57.1	58.2	0.0	9.5	1.081149	37498	37743	402-402	1.000000	71.5
trnI-trnV_p1	trnI-trnV	1	TACCACTGAGCTAATAGCCC	AGTTCAAACCTGATTATCCCT	20	21	50.0	38.1	56.1	54.2	8.9	0.0	6.692317	52621	55578	2994-3000	1.000000	40.2
trnI-trnV_p2	trnI-trnV	2	ACCACTGAGCTAATAGCCC	CAGTTCAAACCTGATTATCCCT	19	22	52.6	40.9	56.2	55.8	8.9	0.0	7.038894	52621	55578	2994-3000	1.000000	40.2
trnI-trnV_p3	trnI-trnV	3	CTACCACTGAGCTAATAGCCC	GTTCAAACCTGATTATCCCT	21	20	52.4	40.0	57.3	52.5	8.9	0.0	7.150518	52621	55578	2994-3000	1.000000	40.2
trnI-trnV_p4	trnI-trnV	4	TACCACTGAGCTAATAGCCC	AGTTCAAACCTGATTATCCC	20	20	50.0	40.0	56.1	52.5	8.9	0.0	7.369940	52621	55578	2994-3000	1.000000	40.2
trnI-trnV_p5	trnI-trnV	5	TACCACTGAGCTAATAGCCC	GTTCAAACCTGATTATCCCT	20	20	50.0	40.0	56.1	52.5	8.9	0.0	7.369940	52621	55578	2993-2999	1.000000	40.2
trnI-ycf2_p1	trnI-ycf2	1	TACCATTCAGCCATGGATGC	GGTTCGTTTCGATAGGGTCC	20	20	50.0	55.0	58.0	58.1	25.4	0.0	0.082621	60885	61528	712-713	1.000000	79.5
trnI-ycf2_p2	trnI-ycf2	2	CCGCGACTTCCCAATTATGA	GGTTCGTTTCGATAGGGTCC	20	20	50.0	55.0	58.1	58.1	8.2	0.0	0.119990	60885	61528	744-745	1.000000	79.1
trnI-ycf2_p3	trnI-ycf2	3	TACCATTCAGCCATGGATGC	ATAGGGTCCATAGGCAACGT	20	20	50.0	50.0	58.0	58.2	25.4	0.0	0.211974	60885	61528	701-702	1.000000	78.4
trnI-ycf2_p4	trnI-ycf2	4	TACCATTCAGCCATGGATGC	TCTGTTTCGGTTCGTTTCGA	20	20	50.0	45.0	58.0	57.8	25.4	0.0	0.221342	60885	61528	720-721	1.000000	78.3
trnI-ycf2_p5	trnI-ycf2	5	CCGCGACTTCCCAATTATGA	ATAGGGTCCATAGGCAACGT	20	20	50.0	50.0	58.1	58.2	8.2	0.0	0.249342	60885	61528	733-734	1.000000	78.1
ycf2_p1	ycf2	1	GAATAAGTTGTATTAACTATGTACTTC	AGCATCTTGTCGCCTTTTCA	27	20	25.9	45.0	52.7	58.1	0.0	0.0	12.371566	61529	62528	1161-1161	1.000000	40.0
ycf2_p2	ycf2	2	GAATAAGTTGTATTAACTATGTACTTC	TGAAAAGCATCTTGTCGCCT	27	20	25.9	45.0	52.7	58.1	0.0	0.0	12.371566	61529	62528	1166-1166	1.000000	40.0
ycf2_p3	ycf2	3	GAATAAGTTGTATTAACTATGTACTTC	GCATCTTGTCGCCTTTTCAA	27	20	25.9	45.0	52.7	57.3	0.0	0.0	12.972511	61529	62528	1160-1160	1.000000	40.0
ycf2_p4	ycf2	4	GAATAAGTTGTATTAACTATGTACTTC	TTGAAAAGCATCTTGTCGCC	27	20	25.9	45.0	52.7	57.3	0.0	0.0	12.972511	61529	62528	1167-1167	1.000000	40.0
ycf2_p5	ycf2	5	GAATAAGTTGTATTAACTATGTACTTC	AAGCATCTTGTCGCCTTTTC	27	20	25.9	45.0	52.7	57.0	0.0	0.0	13.265271	61529	62528	1162-1162	1.000000	40.0
rpl16_p1	rpl16	1	TAACCATCTTGGAGCAGTGC	AGATTTGTTACCTCGGCAGA	20	20	50.0	45.0	57.9	56.6	7.1	0.0	1.557231	73217	74539	1426-1427	1.000000	67.6
rpl16_p2	rpl16	2	TAACCATCTTGGAGCAGTGC	AAGATTTGTTACCTCGGCAGA	20	21	50.0	42.9	57.9	57.3	7.1	0.0	1.809690	73217	74539	1427-1428	1.000000	65.6
rpl16_p3	rpl16	3	TAACCATCTTGGAGCAGTGC	AGATTTGTTACCTCGGCAGAA	20	21	50.0	42.9	57.9	57.3	7.1	0.0	1.809690	73217	74539	1426-1427	1.000000	65.6
rpl16_p4	rpl16	4	TAACCATCTTGGAGCAGTGC	AAGATTTGTTACCTCGGCAGAA	20	22	50.0	40.9	57.9	58.0	7.1	0.0	2.129732	73217	74539	1427-1428	1.000000	63.0
rpl16_p5	rpl16	5	TAACCATCTTGGAGCAGTGC	AGATTTGTTACCTCGGCAGAAA	20	22	50.0	40.9	57.9	58.0	7.1	0.0	2.129732	73217	74539	1426-1427	1.000000	63.0
psaI-ycf4_p1	psaI-ycf4	1	TTACCCTCTGTTTTCGTGCC	GAGCCATTTGGATCGACGAT	20	20	50.0	50.0	58.1	58.1	0.0	10.8	0.242033	83845	84445	721-721	1.000000	78.2
psaI-ycf4_p2	psaI-ycf4	2	CTGTTTTCGTGCCTTTGGTG	GAGCCATTTGGATCGACGAT	20	20	50.0	50.0	58.2	58.1	0.0	10.8	0.290007	83845	84445	714-714	1.000000	77.8
psaI-ycf4_p3	psaI-ycf4	3	TCTGTTTTCGTGCCTTTGGT	GAGCCATTTGGATCGACGAT	20	20	45.0	50.0	58.2	58.1	0.0	10.8	0.371238	83845	84445	715-715	1.000000	77.1
psaI-ycf4_p4	psaI-ycf4	4	TTACCCTCTGTTTTCGTGCC	AGCCATTTGGATCGACGATT	20	20	50.0	45.0	58.1	57.7	0.0	12.4	0.445177	83845	84445	720-720	1.000000	76.5
psaI-ycf4_p5	psaI-ycf4	5	CTGTTTTCGTGCCTTTGGTG	AGCCATTTGGATCGACGATT	20	20	50.0	45.0	58.2	57.7	0.0	12.4	0.493150	83845	84445	713-713	1.000000	76.2
rps12-ycf1_p1	rps12-ycf1	1	CCTTTACGCTGAGGACATCC	ATCTGAGCTGGACCAATTGG	20	20	55.0	50.0	58.1	57.6	0.0	23.3	0.490629	93997	95756	1776-1962	1.000000	80.1
rps12-ycf1_p2	rps12-ycf1	2	ATTGGCTGTCTCGCATTTCT	ATCTGAGCTGGACCAATTGG	20	20	45.0	50.0	57.9	57.6	0.0	23.3	0.551755	93997	95756	1725-1911	1.000000	79.6
rps12-ycf1_p3	rps12-ycf1	3	GCACATACACCCTAGCACAT	ATCTGAGCTGGACCAATTGG	20	20	50.0	50.0	57.7	57.6	0.0	23.3	0.764017	93997	95756	1799-1985	1.000000	77.9
rps12-ycf1_p4	rps12-ycf1	4	ACTCCTTTACGCTGAGGACA	ATCTGAGCTGGACCAATTGG	20	20	50.0	50.0	58.4	57.6	0.0	23.3	0.802911	93997	95756	1779-1965	1.000000	77.6
rps12-ycf1_p5	rps12-ycf1	5	CCTTTACGCTGAGGACATCC	GAGCTGGACCAATTGGCAAT	20	20	55.0	50.0	58.1	58.8	0.0	24.8	0.869812	93997	95756	1772-1958	1.000000	77.1
trnF-accD_p1	trnF-accD	1	GCTAGTATGATGCACAGGGG	GGTTCCACGAGCTCTTTTGA	20	20	55.0	50.0	57.8	58.1	0.0	12.8	0.296925	118188	119938	1909-1910	1.000000	77.7
trnF-accD_p2	trnF-accD	2	GCTAGTATGATGCACAGGGG	CATGGTTCCACGAGCTCTTT	20	20	55.0	50.0	57.8	58.2	0.0	12.8	0.362364	118188	119938	1912-1913	1.000000	77.1
trnF-accD_p3	trnF-accD	3	GTATGATGCACAGGGGAACA	GGTTCCACGAGCTCTTTTGA	20	20	50.0	50.0	57.6	58.1	0.0	12.8	0.539415	118188	119938	1905-1906	1.000000	75.7
trnF-accD_p4	trnF-accD	4	GCTAGTATGATGCACAGGGG	TGGTTCCACGAGCTCTTTTG	20	20	55.0	50.0	57.8	58.4	0.0	12.8	0.582681	118188	119938	1910-1911	1.000000	75.4
trnF-accD_p5	trnF-accD	5	GTATGATGCACAGGGGAACA	CATGGTTCCACGAGCTCTTT	20	20	50.0	50.0	57.6	58.2	0.0	12.8	0.604855	118188	119938	1908-1909	1.000000	75.2