Hotspot

psbA

LSC · 400-1461 · 1062 bp

Back to Cucurbita

Pi 0

MarkerSeek score 25.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	TTTGCATCAACGATTTGGCC	55.0	45.0	58.1	57.9	1887-1892 bp	1.0	79.0
psbA_p2	CAATCCACTGCCTTGATCCA	TTTGCATCAACGATTTGGCC	50.0	45.0	57.9	57.9	1901-1906 bp	1.0	78.9
psbA_p3	AATCCACTGCCTTGATCCAC	TTTGCATCAACGATTTGGCC	50.0	45.0	57.9	57.9	1900-1905 bp	1.0	78.9
psbA_p4	ACAATCCACTGCCTTGATCC	TTTGCATCAACGATTTGGCC	50.0	45.0	57.9	57.9	1902-1907 bp	1.0	78.9
psbA_p5	TTTATATGGGCGAACGACGG	TTTGCATCAACGATTTGGCC	50.0	45.0	58.1	57.9	1948-1953 bp	1.0	78.8