Hotspot

matK

LSC · 2073-3584 · 1512 bp

Back to Cucurbita

Pi 0.00278

MarkerSeek score 58.60

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 9
pi: 0.00278
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CCATTTACATGGTGTCAGAACC	CCTTGTTTTGACTGTATCGCA	45.5	42.9	57.3	56.8	1620 bp	1.0	41.5
matK_p2	CCATTTACATGGTGTCAGAACC	ACCTTGTTTTGACTGTATCGC	45.5	42.9	57.3	56.8	1621 bp	1.0	41.5
matK_p3	CCATTTACATGGTGTCAGAACC	CCTTGTTTTGACTGTATCGCAC	45.5	45.5	57.3	58.2	1620 bp	1.0	41.3
matK_p4	CCATTTACATGGTGTCAGAACC	ACCTTGTTTTGACTGTATCGCA	45.5	40.9	57.3	58.5	1621 bp	1.0	40.4
matK_p5	CCATTTACATGGTGTCAGAACC	TGTTTTGACTGTATCGCACT	45.5	40.0	57.3	55.3	1617 bp	1.0	40.4