Hotspot

atpE

LSC · 56513-56923 · 411 bp

Back to Cucurbita

Pi 0.00292

MarkerSeek score 27.20

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 3
pi: 0.00292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	GCTATAGCCCTTGTGCTTGT	GGAAATACGTTGGGCTAGCA	50.0	50.0	58.0	58.0	986 bp	1.0	80.6
atpE_p2	GCTATAGCCCTTGTGCTTGT	GCACGAGCACGAAAAATTGA	50.0	45.0	58.0	58.0	1059 bp	1.0	80.5
atpE_p3	GCTATAGCCCTTGTGCTTGT	AGCACGAGCACGAAAAATTG	50.0	45.0	58.0	58.0	1060 bp	1.0	80.5
atpE_p4	GCTATAGCCCTTGTGCTTGT	GCAGTTTATGTACCTGCGGA	50.0	50.0	58.0	58.0	1347 bp	1.0	80.4
atpE_p5	TAAATGGGGCACTCGATTCG	GCAGTTTATGTACCTGCGGA	50.0	50.0	58.1	58.0	1528 bp	1.0	80.3