Hotspot

accD

LSC · 61353-62948 · 1596 bp

Back to Cucurbita

Pi 0.00602

MarkerSeek score 51.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1596
variable_sites: 21
pi: 0.00602
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AATAGGGGCAAGAAAGCTCT	AACTACTTGTTCGTCACAAA	45.0	35.0	56.5	53.0	1647-1671 bp	1.0	42.2
accD_p2	AATAGGGGCAAGAAAGCTCT	ACTACTTGTTCGTCACAAAA	45.0	35.0	56.5	53.0	1646-1670 bp	1.0	42.2
accD_p3	AATAGGGGCAAGAAAGCTCT	AACTACTTGTTCGTCACAAAA	45.0	33.3	56.5	53.9	1647-1671 bp	1.0	42.2
accD_p4	AATAGGGGCAAGAAAGCTCT	ACTACTTGTTCGTCACAAAAA	45.0	33.3	56.5	53.9	1646-1670 bp	1.0	42.2
accD_p5	AAATAGGGGCAAGAAAGCTCT	ACTACTTGTTCGTCACAAAA	42.9	35.0	57.3	53.0	1647-1671 bp	1.0	42.2