Hotspot

rpl32-trnL

SSC · 117095-117878 · 784 bp

Back to Cucurbita

Pi 0.00867

MarkerSeek score 60.10

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 784
variable_sites: 15
pi: 0.00867
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	907-937 bp	1.0	80.0
rpl32-trnL_p2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	908-938 bp	1.0	79.9
rpl32-trnL_p3	AGCGTTGAAAGCTTTTTCGC	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.5	58.0	904-934 bp	1.0	79.2
rpl32-trnL_p4	GCGTTGAAAGCTTTTTCGCT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.5	58.0	903-933 bp	1.0	79.2
rpl32-trnL_p5	GCGTTGAAAGCTTTTTCGCT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.5	58.0	904-934 bp	1.0	79.2