Hotspot

atpB-rbcL

LSC · 58417-59210 · 794 bp

Back to Cucurbita

Pi 0.00151

MarkerSeek score 58.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 3
pi: 0.00151
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGAGCGATACGTCCCAAG	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	910-919 bp	1.0	73.2
atpB-rbcL_p2	TTTGAGCGATACGTCCCAAG	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	909-918 bp	1.0	73.0
atpB-rbcL_p3	GATTTGAGCGATACGTCCCA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	912-921 bp	1.0	71.5
atpB-rbcL_p4	TGATTTGAGCGATACGTCCC	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	913-922 bp	1.0	71.5
atpB-rbcL_p5	GATTTGAGCGATACGTCCCA	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.8	58.1	911-920 bp	1.0	71.2