Hotspot

trnM-atpE

LSC · 56344-56512 · 169 bp

Back to Cucurbita

Pi 0.0237

MarkerSeek score 57.90

Primer available yes

Label trnM-atpE

Feature Metadata

label_name: trnM-atpE
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 10
pi: 0.0237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-atpE_p1	CGATGTGATAGGGCCCATTT	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.7	58.0	338 bp	1.0	79.9
trnM-atpE_p2	CGATGTGATAGGGCCCATTT	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.7	58.1	330 bp	1.0	79.7
trnM-atpE_p3	TATCGATGTGATAGGGCCCA	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.7	58.0	341 bp	1.0	79.5
trnM-atpE_p4	ATCGATGTGATAGGGCCCAT	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.3	58.0	340 bp	1.0	79.4
trnM-atpE_p5	TATCGATGTGATAGGGCCCA	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.7	58.1	333 bp	1.0	79.3