Hotspot

atpH-atpI

LSC · 15035-15985 · 951 bp

Back to Cucurbita

Pi 0.0062

MarkerSeek score 66.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 14
pi: 0.0062
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1017-1039 bp	1.0	81.8
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	1102-1124 bp	1.0	81.6
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	987-1009 bp	1.0	81.6
atpH-atpI_p4	GATCCCTTCTACAGCTTGGC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.0	57.8	1072-1094 bp	1.0	81.3
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1018-1040 bp	1.0	80.6