Hotspot

trnR-atpA

LSC · 10983-11538 · 556 bp

Back to Cucurbita

Pi 0.0115

MarkerSeek score 49.80

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 14
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	TGGAATGAAAAGCGTCCATTG	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	706-715 bp	1.0	60.7
trnR-atpA_p2	GGAATGAAAAGCGTCCATTGT	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	705-714 bp	1.0	60.7
trnR-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCCCTTTTGAAAGAAGC	47.6	47.6	57.3	58.0	645-654 bp	1.0	59.9
trnR-atpA_p4	TGGAATGAAAAGCGTCCATTG	TCAGGAACAGATGGAACGTT	42.9	45.0	57.4	56.8	682-691 bp	1.0	59.3
trnR-atpA_p5	TGGAATGAAAAGCGTCCATTG	TTCAGGAACAGATGGAACGT	42.9	45.0	57.4	56.8	683-692 bp	1.0	59.3