Hotspot

rps16-trnQ-UUG

LSC · 6377-7766 · 1390 bp

Back to Cucurbita

Pi 0.00389

MarkerSeek score 62.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1390
variable_sites: 13
pi: 0.00389
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1450-1467 bp	1.0	79.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1461-1478 bp	1.0	79.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GACGACCAACCACTACGAAT	45.0	50.0	58.1	57.6	1546-1563 bp	1.0	77.5
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GACGACCAACCACTACGAAT	50.0	50.0	58.2	57.6	1557-1574 bp	1.0	76.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1454-1471 bp	1.0	75.1