Hotspot

ycf2

LSC · 88737-94603 · 5867 bp

Back to Cucumis

Pi 0.00818

MarkerSeek score 30.50

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 5867
variable_sites: 146
pi: 0.00818
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 30.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GAAGAAGGGGAAGGAGTCCT	ATTTCATCCGGGAAAAGCCA	55.0	45.0	58.0	57.8	563-684 bp	1.0	86.4
ycf2_p2	GAAGAAGGGGAAGGAGTCCT	TTTCATCCGGGAAAAGCCAT	55.0	45.0	58.0	57.8	562-683 bp	1.0	86.3
ycf2_p3	GAAGAAGGGGAAGGAGTCCT	TTCATCCGGGAAAAGCCATC	55.0	50.0	58.0	58.2	561-682 bp	1.0	86.2
ycf2_p4	GAAGAAGGGGAAGGAGTCCT	CATCCGGGAAAAGCCATCTT	55.0	50.0	58.0	58.2	559-680 bp	1.0	86.0
ycf2_p5	TCTATCCGGACCCGATGAAA	TTTCATCCGGGAAAAGCCAT	50.0	45.0	57.9	57.8	824-945 bp	1.0	85.0