Hotspot

ycf1

SSC · 125236-130870 · 5635 bp

Back to Cucumis

Pi 0.0357

MarkerSeek score 56.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5635
variable_sites: 607
pi: 0.0357
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAGAGACAGACGAACGGGTT	AGGGCGATATACGAGTCTGG	50.0	55.0	58.1	58.5	803 bp	0.181818	42.9
ycf1_p2	TTAGAGACAGACGAACGGGT	AGGGCGATATACGAGTCTGG	50.0	55.0	58.1	58.5	804 bp	0.181818	42.9
ycf1_p3	TAGAGACAGACGAACGGGTT	GGGCGATATACGAGTCTGGA	50.0	55.0	58.1	58.5	802 bp	0.181818	42.8
ycf1_p4	TTAGAGACAGACGAACGGGT	GGGCGATATACGAGTCTGGA	50.0	55.0	58.1	58.5	803 bp	0.181818	42.8
ycf1_p5	TCTCCGACCTCTAGGGGATA	AGGGCGATATACGAGTCTGG	55.0	55.0	57.9	58.5	1022 bp	0.181818	42.6