Hotspot

accD

LSC · 59670-61241 · 1572 bp

Back to Cucumis

Pi 0.0164

MarkerSeek score 43.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1572
variable_sites: 79
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CTGGATCCGCAATTAAGCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2598-2714 bp	1.0	86.7
accD_p2	CTGGATCCGCAATTAAGCCT	GAGGGTAAGGTGGTGAGAGT	50.0	55.0	58.0	58.1	2551-2667 bp	1.0	86.5
accD_p3	CTGGATCCGCAATTAAGCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	2604-2720 bp	1.0	86.1
accD_p4	TGTGCTGGATCCGCAATTAA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	2602-2718 bp	1.0	85.9
accD_p5	TTGTGCTGGATCCGCAATTA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	2603-2719 bp	1.0	85.9