Hotspot

ndhB-trnL-CAA

IRa · 145254-145883 · 630 bp

Back to Cucumis

Pi 0.00172

MarkerSeek score 46.00

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 630
variable_sites: 3
pi: 0.00172
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	CCAGGAATATCAATGAACCCGA	TTCAACGGGCATTCTCAACA	45.5	45.0	57.9	58.0	617-736 bp	1.0	67.5
ndhB-trnL-CAA_p2	ACCAGGAATATCAATGAACCCG	TTCAACGGGCATTCTCAACA	45.5	45.0	58.2	58.0	618-737 bp	1.0	66.7
ndhB-trnL-CAA_p3	CCAGGAATATCAATGAACCCGA	TCAACGGGCATTCTCAACAT	45.5	45.0	57.9	57.8	616-735 bp	1.0	66.1
ndhB-trnL-CAA_p4	CCAGGAATATCAATGAACCCGA	TCCAATTCAACGGGCATTCT	45.5	45.0	57.9	57.8	622-741 bp	1.0	65.9
ndhB-trnL-CAA_p5	TGAAATTGCTCAGGATACCCT	TTCAACGGGCATTCTCAACA	42.9	45.0	56.7	58.0	591-710 bp	1.0	65.7