Hotspot

psbZ-trnG-UCC

LSC · 38052-38512 · 461 bp

Back to Cucumis

Pi 0.0177

MarkerSeek score 48.10

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 32
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	AGTCTTTCTGGTGGGTATCCT	AATCGAACCCGCATCTTCTC	47.6	50.0	57.8	58.1	559-583 bp	1.0	75.7
psbZ-trnG-UCC_p2	AGTCTTTCTGGTGGGTATCCT	GAATCGAACCCGCATCTTCT	47.6	50.0	57.8	58.1	560-584 bp	1.0	75.7
psbZ-trnG-UCC_p3	AGTCTTTCTGGTGGGTATCCT	CATTATCCTCATCCTGGGCG	47.6	55.0	57.8	57.9	588-612 bp	1.0	75.0
psbZ-trnG-UCC_p4	AGTCTTTCTGGTGGGTATCCT	CCATTATCCTCATCCTGGGC	47.6	55.0	57.8	57.5	589-613 bp	1.0	71.8
psbZ-trnG-UCC_p5	GTCTTTCTGGTGGGTATCCT	AATCGAACCCGCATCTTCTC	50.0	50.0	56.2	58.1	558-582 bp	1.0	71.1