primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.241837	8750	9455	775-855	1.000000	73.8
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.243225	8750	9455	774-854	1.000000	73.8
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.248863	8750	9455	775-855	1.000000	73.7
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.250251	8750	9455	774-854	1.000000	73.7
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.2	58.2	0.0	1.2	1.388603	8750	9455	829-909	1.000000	72.4
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AGCCTTCCAAGCTAACGATG	CGAAGCTTATCCGGGAGATG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.101527	10530	11064	1556-1580	1.000000	84.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	ACTAGAGACGTCGACATCGT	CGAAGCTTATCCGGGAGATG	20	20	50.0	55.0	58.0	57.9	17.2	0.0	0.079049	10530	11064	1894-1914	1.000000	83.6
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	ACTAGAGACGTCGACATCGT	GATCCCTCCAAACAAGCACA	20	20	50.0	50.0	58.0	58.1	17.2	0.0	0.097661	10530	11064	1959-1979	1.000000	83.3
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	ACTAGAGACGTCGACATCGT	TGATCCCTCCAAACAAGCAC	20	20	50.0	50.0	58.0	58.1	17.2	0.0	0.097661	10530	11064	1960-1980	1.000000	83.3
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	ACTAGAGACGTCGACATCGT	CCTGAAGCGGAAGTCCTTTT	20	20	50.0	50.0	58.0	58.1	17.2	0.0	0.109800	10530	11064	1304-1316	0.181818	46.7
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	AGTCTTTCTGGTGGGTATCCT	AATCGAACCCGCATCTTCTC	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.265719	38052	38512	559-583	1.000000	75.7
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	AGTCTTTCTGGTGGGTATCCT	GAATCGAACCCGCATCTTCT	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.265719	38052	38512	560-584	1.000000	75.7
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	AGTCTTTCTGGTGGGTATCCT	CATTATCCTCATCCTGGGCG	21	20	47.6	55.0	57.8	57.9	0.0	0.0	1.312050	38052	38512	588-612	1.000000	75.0
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	AGTCTTTCTGGTGGGTATCCT	CCATTATCCTCATCCTGGGC	21	20	47.6	55.0	57.8	57.5	0.0	0.0	1.710939	38052	38512	589-613	1.000000	71.8
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	GTCTTTCTGGTGGGTATCCT	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	56.2	58.1	0.0	0.0	1.832254	38052	38512	558-582	1.000000	71.1
psaA-ycf3_p1	psaA-ycf3	1	GTTCTCGAGAAATGACCCGG	ATCAAGCCGCTGAGTATTGG	20	20	55.0	50.0	58.4	58.0	28.6	0.0	0.399381	43916	44628	915-927	1.000000	78.8
psaA-ycf3_p2	psaA-ycf3	2	TGTTCTCGAGAAATGACCCG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.6	58.0	28.6	0.0	0.404746	43916	44628	916-928	1.000000	78.7
psaA-ycf3_p3	psaA-ycf3	3	GTTCTCGAGAAATGACCCGG	AGCCGCTGAGTATTGGAAAC	20	20	55.0	50.0	58.4	58.3	28.6	0.0	0.619356	43916	44628	911-923	1.000000	77.0
psaA-ycf3_p4	psaA-ycf3	4	GTTCTCGAGAAATGACCCGG	CAAGCCGCTGAGTATTGGAA	20	20	55.0	50.0	58.4	58.3	28.6	0.0	0.620585	43916	44628	913-925	1.000000	77.0
psaA-ycf3_p5	psaA-ycf3	5	TGTTCTCGAGAAATGACCCG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.6	58.3	28.6	0.0	0.624721	43916	44628	912-924	1.000000	77.0
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TTCCGGTTTTGGGCCTTTTA	CTCAGAGGTTAGAGCATCGC	20	20	45.0	55.0	57.9	57.9	4.5	0.0	0.216368	48231	48579	499-520	1.000000	81.7
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TTCCGGTTTTGGGCCTTTTA	ATGGTCATCGGTTCGATTCC	20	20	45.0	50.0	57.9	57.8	4.5	0.0	0.324122	48231	48579	467-488	1.000000	81.0
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.3	57.9	9.5	0.0	0.409520	48231	48579	438-459	1.000000	80.6
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TTCCGGTTTTGGGCCTTTTA	CTGTTATGTGAGCCCGCTTA	20	20	45.0	50.0	57.9	57.7	4.5	0.0	0.393156	48231	48579	520-541	1.000000	80.1
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTCCGGTTTTGGGCCTTTTA	TGTTATGTGAGCCCGCTTAG	20	20	45.0	50.0	57.9	57.7	4.5	0.0	0.393156	48231	48579	519-540	1.000000	80.1
accD_p1	accD	1	CTGGATCCGCAATTAAGCCT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	7.4	26.9	0.054131	59670	61241	2598-2714	1.000000	86.7
accD_p2	accD	2	CTGGATCCGCAATTAAGCCT	GAGGGTAAGGTGGTGAGAGT	20	20	50.0	55.0	58.0	58.1	7.4	0.0	0.087148	59670	61241	2551-2667	1.000000	86.5
accD_p3	accD	3	CTGGATCCGCAATTAAGCCT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	7.4	26.8	0.130665	59670	61241	2604-2720	1.000000	86.1
accD_p4	accD	4	TGTGCTGGATCCGCAATTAA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.9	58.0	25.8	26.9	0.148295	59670	61241	2602-2718	1.000000	85.9
accD_p5	accD	5	TTGTGCTGGATCCGCAATTA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.9	58.0	25.8	26.9	0.148295	59670	61241	2603-2719	1.000000	85.9
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	GAAATGTCACCAAATCGCCC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.391887	71306	72095	946-947	1.000000	78.3
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	GAAATGTCACCAAATCGCCC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.391887	71306	72095	945-946	1.000000	78.3
rpl20-rps12_p3	rpl20-rps12	3	TTTGTTCTACGTCTCCGAGC	GAAATGTCACCAAATCGCCC	20	20	50.0	50.0	57.7	57.7	0.0	0.0	0.620936	71306	72095	904-905	1.000000	76.5
rpl20-rps12_p4	rpl20-rps12	4	TGTTCTACGTCTCCGAGCTA	GAAATGTCACCAAATCGCCC	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.669642	71306	72095	902-903	1.000000	76.2
rpl20-rps12_p5	rpl20-rps12	5	TTGTTCTACGTCTCCGAGCT	GAAATGTCACCAAATCGCCC	20	20	50.0	50.0	58.5	57.7	0.0	0.0	0.746604	71306	72095	903-904	1.000000	75.5
clpP_p1	clpP	1	ATGGTCTAAACGAGTCGCAC	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.026547	72361	74089	2560-2822	1.000000	83.8
clpP_p2	clpP	2	GGTCTAAACGAGTCGCACAT	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.026547	72361	74089	2558-2820	1.000000	83.8
clpP_p3	clpP	3	CATGGTCTAAACGAGTCGCA	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.027918	72361	74089	2561-2823	1.000000	83.8
clpP_p4	clpP	4	ACTTTCTTTTGTCCCGGTCC	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041661	72361	74089	2580-2842	1.000000	83.6
clpP_p5	clpP	5	CTTTCTTTTGTCCCGGTCCA	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043057	72361	74089	2579-2841	1.000000	83.6
ycf2_p1	ycf2	1	GAAGAAGGGGAAGGAGTCCT	ATTTCATCCGGGAAAAGCCA	20	20	55.0	45.0	58.0	57.8	5.6	9.5	0.270420	94351	94470	563-684	1.000000	86.4
ycf2_p2	ycf2	2	GAAGAAGGGGAAGGAGTCCT	TTTCATCCGGGAAAAGCCAT	20	20	55.0	45.0	58.0	57.8	5.6	0.0	0.270420	94351	94470	562-683	1.000000	86.3
ycf2_p3	ycf2	3	GAAGAAGGGGAAGGAGTCCT	TTCATCCGGGAAAAGCCATC	20	20	55.0	50.0	58.0	58.2	5.6	0.0	0.277583	94351	94470	561-682	1.000000	86.2
ycf2_p4	ycf2	4	GAAGAAGGGGAAGGAGTCCT	CATCCGGGAAAAGCCATCTT	20	20	55.0	50.0	58.0	58.2	5.6	0.0	0.272532	94351	94470	559-680	1.000000	86.0
ycf2_p5	ycf2	5	TCTATCCGGACCCGATGAAA	TTTCATCCGGGAAAAGCCAT	20	20	50.0	45.0	57.9	57.8	6.1	0.0	0.306337	94351	94470	824-945	1.000000	85.0
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCGA	20	22	50.0	45.5	58.0	57.9	5.8	0.0	2.093188	95621	96198	545-664	1.000000	67.6
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCGA	20	22	50.0	45.5	58.0	57.9	5.8	0.0	2.094558	95621	96198	543-662	1.000000	67.6
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	TTCAACGGGCATTCTCAACA	CCAGGAATATCAATGAACCCGA	20	22	45.0	45.5	58.0	57.9	0.0	0.0	2.111092	95621	96198	617-736	1.000000	67.5
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCGA	20	22	50.0	45.5	58.1	57.9	0.0	0.0	2.167485	95621	96198	533-652	1.000000	67.0
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	GTCTCGCGTGTCTACCATTT	ACCAGGAATATCAATGAACCCG	20	22	50.0	45.5	58.0	58.2	5.8	0.0	2.187273	95621	96198	546-665	1.000000	66.9
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	TGCCCTTTTCTGACGAAACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.053392	115603	115706	308-311	1.000000	82.9
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	TGCCCTTTTCTGACGAAACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.054762	115603	115706	307-310	1.000000	82.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GAGAAAGCCGCTATGGTGAA	TGCCCTTTTCTGACGAAACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.069737	115603	115706	323-326	1.000000	82.6
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	ATGATTTCGAGAAAGCCGCT	TGCCCTTTTCTGACGAAACA	20	20	45.0	45.0	58.0	58.0	10.7	0.0	0.075400	115603	115706	331-334	1.000000	82.5
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTGAAATCGGTAGACACGCT	AAATAGTACTTGGCGCGGTT	20	20	50.0	45.0	58.0	57.9	0.0	8.8	0.111367	115603	115706	503-506	1.000000	82.0
ycf1_p1	ycf1	1	TAGAGACAGACGAACGGGTT	AGGGCGATATACGAGTCTGG	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.562576	125888	126007	803-803	0.181818	42.9
ycf1_p2	ycf1	2	TTAGAGACAGACGAACGGGT	AGGGCGATATACGAGTCTGG	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.562576	125888	126007	804-804	0.181818	42.9
ycf1_p3	ycf1	3	TAGAGACAGACGAACGGGTT	GGGCGATATACGAGTCTGGA	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.566987	125888	126007	802-802	0.181818	42.8
ycf1_p4	ycf1	4	TTAGAGACAGACGAACGGGT	GGGCGATATACGAGTCTGGA	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.566987	125888	126007	803-803	0.181818	42.8
ycf1_p5	ycf1	5	TCTCCGACCTCTAGGGGATA	AGGGCGATATACGAGTCTGG	20	20	55.0	55.0	57.9	58.5	18.0	0.0	0.593366	125888	126007	1022-1022	0.181818	42.6
trnN-GUU_p1	trnN-GUU	1	CCGTTGTTCCTCAGTAGCTC	GGGAACGGGTTACCTACTCT	20	20	55.0	55.0	58.0	58.1	0.0	5.5	0.144366	131299	131370	155-155	1.000000	80.9
trnN-GUU_p2	trnN-GUU	2	TGGGTTGAGAACGGGAATTG	GGGAACGGGTTACCTACTCT	20	20	50.0	55.0	58.1	58.1	0.0	5.5	0.237749	131299	131370	196-196	1.000000	79.7
trnN-GUU_p3	trnN-GUU	3	TTGGTCATGGGTTGAGAACG	GGGAACGGGTTACCTACTCT	20	20	50.0	55.0	58.1	58.1	0.0	5.5	0.251159	131299	131370	203-203	1.000000	79.6
trnN-GUU_p4	trnN-GUU	4	CATGGGTTGAGAACGGGAAT	GGGAACGGGTTACCTACTCT	20	20	50.0	55.0	57.9	58.1	0.0	5.5	0.270806	131299	131370	198-198	1.000000	79.4
trnN-GUU_p5	trnN-GUU	5	CCGTTGTTCCTCAGTAGCTC	AACAAAGACACAGGGAACGG	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.326779	131299	131370	167-167	1.000000	79.3
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	CCAGGAATATCAATGAACCCGA	TTCAACGGGCATTCTCAACA	22	20	45.5	45.0	57.9	58.0	0.0	0.0	2.111092	145254	145883	617-736	1.000000	67.5
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	ACCAGGAATATCAATGAACCCG	TTCAACGGGCATTCTCAACA	22	20	45.5	45.0	58.2	58.0	0.0	0.0	2.205178	145254	145883	618-737	1.000000	66.7
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	CCAGGAATATCAATGAACCCGA	TCAACGGGCATTCTCAACAT	22	20	45.5	45.0	57.9	57.8	0.0	0.0	2.284184	145254	145883	616-735	1.000000	66.1
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	CCAGGAATATCAATGAACCCGA	TCCAATTCAACGGGCATTCT	22	20	45.5	45.0	57.9	57.8	0.0	0.0	2.299733	145254	145883	622-741	1.000000	65.9
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TGAAATTGCTCAGGATACCCT	TTCAACGGGCATTCTCAACA	21	20	42.9	45.0	56.7	58.0	0.0	0.0	2.337383	145254	145883	591-710	1.000000	65.7