Hotspot

ndhF

SSC · 111294-113513 · 2220 bp

Back to Ctenium

Pi 0.0144

MarkerSeek score 54.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 80
pi: 0.0144
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGTTTGGTGAATCGGAAACA	TGCATTTTGATGAGCCTCGT	42.9	45.0	58.1	58.2	2342 bp	1.0	73.0
ndhF_p2	AGGTTTGGTGAATCGGAAACA	GTTTGCTGGCTTATTTGGCA	42.9	45.0	58.1	57.5	2480-2500 bp	1.0	70.6
ndhF_p3	TGGGCAGATAAAACGAAGGT	TGCATTTTGATGAGCCTCGT	45.0	45.0	57.1	58.2	2657-2848 bp	0.833333	70.4
ndhF_p4	TGGGCAGATAAAACGAAGGT	GTTTGCTGGCTTATTTGGCA	45.0	45.0	57.1	57.5	2795-2986 bp	0.833333	68.1
ndhF_p5	TGGGCAGATAAAACGAAGGT	CGTTTGCTGGCTTATTTGGC	45.0	50.0	57.1	58.7	2796-2987 bp	0.833333	66.5