Hotspot

rpl32-ndhF

SSC · 110396-111293 · 898 bp

Back to Ctenium

Pi 0.034

MarkerSeek score 76.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 898
variable_sites: 71
pi: 0.034
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGACATCGAAGTACGTTTTT	GGAAGTGGGAGAAGAGGGAT	35.0	55.0	53.2	58.1	790-984 bp	1.0	51.1
rpl32-ndhF_p2	TTGACATCGAAGTACGTTTT	GGAAGTGGGAGAAGAGGGAT	35.0	55.0	53.2	58.1	791-985 bp	1.0	51.1
rpl32-ndhF_p3	TTTGACATCGAAGTACGTTT	GGAAGTGGGAGAAGAGGGAT	35.0	55.0	53.2	58.1	792-986 bp	1.0	51.1
rpl32-ndhF_p4	TTTTGACATCGAAGTACGTT	GGAAGTGGGAGAAGAGGGAT	35.0	55.0	53.2	58.1	793-987 bp	1.0	51.1
rpl32-ndhF_p5	TTTTTGACATCGAAGTACGT	GGAAGTGGGAGAAGAGGGAT	35.0	55.0	53.2	58.1	794-988 bp	1.0	51.1