Hotspot

trnL-UAG-rpl32

SSC · 109701-110230 · 530 bp

Back to Ctenium

Pi 0.0287
MarkerSeek score 70.20
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
530
variable_sites
39
pi
0.0287
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA ACCAAAAGGTTTTTCTGGGT 50.0 40.0 57.9 55.5 580-594 bp 1.0 67.1
trnL-UAG-rpl32_p2 AGCAGCGTGTCTACCAATTT ACCAAAAGGTTTTTCTGGGT 45.0 40.0 57.8 55.5 573-587 bp 1.0 66.7
trnL-UAG-rpl32_p3 CCTAAGAGCAGCGTGTCTAC ACCAAAAGGTTTTTCTGGGT 55.0 40.0 57.8 55.5 579-593 bp 1.0 66.5
trnL-UAG-rpl32_p4 CTAAGAGCAGCGTGTCTACC ACCAAAAGGTTTTTCTGGGT 55.0 40.0 57.8 55.5 578-592 bp 1.0 66.5
trnL-UAG-rpl32_p5 GCTTGAGCACTGCTTCCTAA ACCAAAAGGTTTTTCTGGGT 50.0 40.0 58.2 55.5 594-608 bp 1.0 66.4