Hotspot

ndhD-ccsA

SSC · 108391-108574 · 184 bp

Back to Ctenium

Pi 0.0412

MarkerSeek score 68.90

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 184
variable_sites: 19
pi: 0.0412
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	GCGGTTTTGTTGCGGAATTA	GCAACTCTTTTGTGCGGATC	45.0	50.0	57.9	58.0	1037-1049 bp	1.0	83.5
ndhD-ccsA_p2	GCGGTTTTGTTGCGGAATTA	ACTGGTACCTGCTCTCCAAT	45.0	50.0	57.9	58.0	1101-1113 bp	1.0	83.2
ndhD-ccsA_p3	GCGGTTTTGTTGCGGAATTA	ACCTGCTCTCCAATCTCAGT	45.0	50.0	57.9	58.1	1095-1107 bp	1.0	83.1
ndhD-ccsA_p4	GCGGTTTTGTTGCGGAATTA	GAAGGGTACGAATTCTGCGT	45.0	50.0	57.9	58.0	636-648 bp	0.833333	76.1
ndhD-ccsA_p5	TGCAGCTTCAACCTCTCTTG	GAAGGGTACGAATTCTGCGT	50.0	50.0	58.1	58.0	941-953 bp	0.833333	74.8