Hotspot

infA-rps8

LSC · 75985-76063 · 79 bp

Back to Ctenium

Pi 0.0743

MarkerSeek score 64.30

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 17
pi: 0.0743
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ACTGGACCGGATTTTTCCTG	AAAGCGTATAAGCCGACCTG	50.0	50.0	57.8	58.1	410 bp	0.833333	76.3
infA-rps8_p2	GGGTAGGGCTTCCGTAACTA	AAAGCGTATAAGCCGACCTG	55.0	50.0	58.2	58.1	335 bp	0.833333	76.3
infA-rps8_p3	GAACATTCCGTTGGGTAGGG	AAAGCGTATAAGCCGACCTG	55.0	50.0	58.3	58.1	347 bp	0.833333	75.9
infA-rps8_p4	ACGCTTCGAATCCTTATGGG	AAAGCGTATAAGCCGACCTG	50.0	50.0	57.8	58.1	515 bp	0.833333	75.1
infA-rps8_p5	ATGACTCTAATTCGCCCACG	AAAGCGTATAAGCCGACCTG	50.0	50.0	57.8	58.1	749 bp	0.833333	74.5