Hotspot

psbE-petL

LSC · 61744-62941 · 1198 bp

Back to Ctenium

Pi 0.0194

MarkerSeek score 66.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 64
pi: 0.0194
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	ACGCAGTCACGAATAATCCC	45.0	50.0	58.0	58.1	1632-1671 bp	1.0	86.8
psbE-petL_p2	CAAGTGGTCGTGCTAGAGAC	ACGCAGTCACGAATAATCCC	55.0	50.0	58.0	58.1	2137-2176 bp	1.0	86.2
psbE-petL_p3	CAAGTGGTCGTGCTAGAGAC	CAAAACCCGATGTCGTAGGT	55.0	50.0	58.0	57.9	2331-2371 bp	1.0	86.0
psbE-petL_p4	CAGCTAATCCGTGAACAGCT	ACGCAGTCACGAATAATCCC	50.0	50.0	58.0	58.1	1843-1882 bp	0.833333	75.7
psbE-petL_p5	CAGCTAATCCGTGAACAGCT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	2037-2077 bp	0.833333	75.3