Hotspot

petA-psbJ

LSC · 60068-60974 · 907 bp

Back to Ctenium

Pi 0.0228

MarkerSeek score 56.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 55
pi: 0.0228
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCACAAGTCTTTTTGGTTCTCA	GGTTCGTATTCTGGATTGGGT	40.9	47.6	57.6	57.7	997-1010 bp	1.0	56.4
petA-psbJ_p2	AGCACAAGTCTTTTTGGTTCTC	GGTTCGTATTCTGGATTGGGT	40.9	47.6	57.4	57.7	998-1011 bp	1.0	54.2
petA-psbJ_p3	AGCACAAGTCTTTTTGGTTCT	GGTTCGTATTCTGGATTGGGT	38.1	47.6	56.2	57.7	998-1011 bp	1.0	52.9
petA-psbJ_p4	GCACAAGTCTTTTTGGTTCTC	GGTTCGTATTCTGGATTGGGT	42.9	47.6	55.9	57.7	997-1010 bp	1.0	50.7
petA-psbJ_p5	GCACAAGTCTTTTTGGTTCTCA	GGTTCGTATTCTGGATTGGG	40.9	50.0	57.6	55.9	997-1010 bp	1.0	49.8