Hotspot

ndhC-trnV-UAC

LSC · 49630-50765 · 1136 bp

Back to Ctenium

Pi 0.0206
MarkerSeek score 62.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1136
variable_sites
64
pi
0.0206
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGGAACA GGTTCGAATCCGTATAGCCC 40.9 55.0 57.8 57.9 844-1202 bp 1.0 70.3
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGGAACA CGGTTCGAATCCGTATAGCC 40.9 55.0 57.8 58.3 845-1203 bp 1.0 68.5
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGGAACA GGTTCGAATCCGTATAGCCC 42.9 55.0 56.3 57.9 843-1201 bp 1.0 66.7
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGGAACA CGGTTCGAATCCGTATAGCC 42.9 55.0 56.3 58.3 844-1202 bp 1.0 64.9
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGGAAC GGTTCGAATCCGTATAGCCC 42.9 55.0 56.1 57.9 844-1202 bp 1.0 64.5