Hotspot

trnF-GAA-ndhJ

LSC · 47390-47951 · 562 bp

Back to Ctenium

Pi 0.028

MarkerSeek score 66.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 40
pi: 0.028
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTCTGGGTTTGGAGAAGTGC	50.0	50.0	58.1	58.0	768-792 bp	1.0	84.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGAGGGGTTCTTTTGTGCAG	50.0	50.0	58.1	58.0	1174-1200 bp	0.833333	74.8
trnF-GAA-ndhJ_p3	ATGCATAGGACTCTCCCCTC	AGAGGGGTTCTTTTGTGCAG	55.0	50.0	58.0	58.0	1328-1329 bp	0.5	60.1
trnF-GAA-ndhJ_p4	ATGCATAGGACTCTCCCCTC	GAGGGGTTCTTTTGTGCAGA	55.0	50.0	58.0	58.0	1327-1328 bp	0.5	60.1
trnF-GAA-ndhJ_p5	ATGCATAGGACTCTCCCCTC	TTCTGGGTTTGGAGAAGTGC	55.0	50.0	58.0	58.0	926-927 bp	0.5	60.1